Journal ID : TRKU-01-10-2020-11184
[This article belongs to Volume - 62, Issue - 09]
Total View : 424

Title : THE EXTRACTION OF FLAVONOIDS FROM GEDI LEAVES (Abelmoschus manihot L.) USING ULTRASONIC METHODS AND ANALYSIS OF ANTIOXIDANT EFFECTIVENESS

Abstract :

Gedi (Abelmoschus manihot L.) is a tropical plant in the Malvacea family that is used as food and medicinal/herbal plant? This shrub can be found in Sulawesi, Maluku, Nusa Tenggara Barat dan Papua. The objective of this study was to determine the parameters to obtain an optimal extraction yield. As parts of ultrasonic extraction method, the parameters tested were ratios of dried Gedi leaf powder to ethanol and time of sonication. Gedi leaves from North Sulawesi was dried in an oven at 70oC for five hours and followed by grinding and sieving with 40-mesh siever. The first phase of the study was testing different solution ratios (m/v) of 1:10, 1:20, 1:30, 1:40, and 1:50 at a constant time of extraction of 30 minutes. The second phase was varying the time of sonication at 5, 10, 15, 20, and 30 minutes at the constant solution ratio of 1:40 (m/v). Throughout the study, the ultrasonic wavelength was set at 40 kHz and the temperature on the water bath for concentrating the Gedi leaf extract was set at 50 - 60oC. The optimal yields from both phases were 9.866% for the parameters of 1:40 (m/v) ratio and 30-minute sonication. Further analyses of Gedi leaf extract produced antioxidant effectiveness of 86.77% and phenolic content of 24.34 𝜇g/mg

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