The purpose of this study is to determine the distribution of total coliform and Escherichia coli as a biological indicator within groundwater, located in Kebayoran Baru, South Jakarta, Indonesia (6° 14' 37.039" S 106° 48' 0.504" E). Total coliform and Escherichia coli are bacteria whose presence can be indicated from contaminated water by human faeces or animal waste. The method used for sampling was done by stratified random sampling techniques. In this random sample of stratification, it is divided within several segments based on the categories. Sampling is divided according to area and each sample is collected based on income which are divided into three groups, namely, high income, medium income and low income as many as 24 sampling points. The presence of total coliform and E. coli was calculated using Most Probable Number method and ArcGIS Program to process bacterial distribution pattern. The results of this study showed that in all groundwater samples containing coliform and E. coli, it was only found within sampling points that ranged from 6 MPN/100 ml up to 1100 MPN/100 ml. Whereas E. coli bacteria in ground water ranged from 0 MPN/100 ml up to 263 MPN/100 ml are not found