This research aims to produce a valid and reliable Mathematics Assessment Instrument in the form of HOTS test question Based on Aceh Local Wisdom and to describe the quality of HOTS test question to measure higher order thinking skill of Grade VI elementary school students. This research was a research and development adapted from research development of formative research Tessmer type namely the preliminary and formation evaluation stage consist of self evaluation, prototyping (expert review, one-to-one, and small group), and field test. The subjects on one to one were 3 grade VI students of SDN Peunaga Cut Ujong West Aceh, the subjects in small group were 6 grade VI students of SDN Peunaga Cut Ujong West Aceh, and the subjects in the field test were 30 grade VI students of Peureumee West Aceh. The research results show that HOTS assessment instrument Aceh Local Wisdom Based in the form of test questions consisting of 15 essay question items from the material aspect, construction, and language is stated valid and feasible to be used. The instrument has reliability coefficient in the amount of 0,85%, the difficulty level average 0,42 (medium), and distinguishing power 0,31 (accepted without revision). From the field test, the average result of students’ HOTS Aceh Local Wisdom Based is 78,08 with medium category, there are 7 students (23,33%) owning medium HOTS, 20 students (66,67%) have high HOTS, and 3 students (10%) have extremely high HOTS