Oreochromis niloticus is one of the freshwater aquaculture fish which has high economic value. Current cultivation of tilapia still facing the problem of disease that can reduce the quality of the production. One common type of fish disease in tilapia cultivation is parasites. Analysis of ectoparasites prevalence and intensity was conducted in biofloc system cultivation of tilapia fish in Sukabumi Regency, West Java, Indonesia. The results showed that the types of ectoparasites in tilapia cultivated with biofloc systems were Trichodina sp., Glossatella sp., Dactyrogylus sp., and Gyrodactylus sp. with a prevalence ranging from 10-60% and intensities ranging from 1-3 individuals/fish. This parasitic infection was categorized as infection often to very often with low intensity of infection. The fish gill is the major targeted organ by parasite infections. Understanding the prevalence and intensity of the parasites infection in tilapia cultivation may benefit the community to prepare future action in handling this matter.