Increased population and urbanization is one of the changes in the use of the Wadaslintang Reservoir catchment area, this condition has the potential to cause pollution of some heavy metals that can accumulate in this aquatic biota. This study aims to determine the concentration and bioconcentration of Pb, Zn, Cu, and Cd heavy metals in Anodonta woodiana Lea (freshwater shellfish), Pomacea canaliculata Lamarck (golden apple snail), and Cherax quadricarinatus Von Martens (crayfish) in the Wadaslintang Reservoir. Samples of freshwater shellfish, snails, and crayfish were randomly collected in an area of + 100 m2 in three research stations at a depth of 0.0 - 0.5 m. Sampling was conducted in August - October 2018. The results showed that the three aquatic organisms contained heavy metals Cu, Cd, Pb, and Zn. The highest concentration is in A. woodiana, then P. canaliculata and the lowest concentration is in C. quadricarinatus. The results of the BCF value calculation showed the highest bioaccumulation values found in A. woodiana and P. canaliculata were Zn, which included in the category of moderate accumulation. Whereas in C. quadricarinatus the highest bioaccumulation value is Pb, but it is still included in the low accumulation category