Colonization of the oral cavity by potentially pathogenic bacteria constitutes threatens its transmission to different human tissues and organs, this study provides isolation and identification of 26 isolates (14 Klebsiella pneumoniae and 12 Enterobacter cloacae) recovered from oral cavity infections. In this study, some of virulence factors were detected by traditional phenotypic methods. The results showed 9 (64.28%) of 14 K. pneumoniae isolates demonstrated hypermucoviscosity. The results showed that 57.1% (8/14), 58.4% (7/12) and of isolates were encapsulated K. pneumoniae and E. cloacae isolates, respectively. In this study, there were 78.5 %(11/14) of K. pneumoniae and 66.6% (8/12) of E. cloacae isolates showed their ability to adhere with epithelial cell. The results in of biofilm formation were indicated according to (Salwa et al., 2011) in which 71.4% (10/14) of K. pneumoniae and 83.3% (10/12) of E. cloacae isolates appeared high biofilm formation (strong positive adherence). While 28.6% (4/14) K. pneumoniae and 16.7% (2/12) of E. cloacae isolates appeared moderate biofilm formation