Journal ID : TRKU-07-04-2020-10653
[This article belongs to Volume - 62, Issue - 03]
Total View : 191

Title : Nano emulsion of kepok banana leaves extract improving antioxidant and antibacterial activity

Abstract :

The ethyl acetate extract of Musa paradisiaca L. leaves contains quercetin. The main problem of quercetin associated with oral administration is its poor aqueous solubility. Nano emulsion is drug delivery system that it was prove to enhancement the solubility and permeation of lipophilic drugs. The research aimed to investigate nano emulsion as carrier of extract to improve the activity of antioxidant and antibacterial. The nano emulsion of extract was formulated by various concentration of Tween-80 and PEG-400, and it was optimized by Factorial Design on Design Expert®7 software based on desirability value. Design using response droplet size, zeta potential, and pH. The optimum formula selected was tested for physical stability and activity. Physical stability was tested by centrifugation and cyling test methods. The antioxidant and antibacterial activity of fo and extract were determined by DPPH and Kirby Bauer methods, respectively, and its analyzed by independent T-test. The optimum formula was obtained by 15% Tween-80 and 10% PEG-400 with desirability value 82,4%. It has pH 6.4, droplet size 15.3 nm, a polydisperse index (PI) 0.012, a zeta potential -23.3 mV. Physical stability test shows the formula does not experience syneresis, separation, creaming or cracking. The independent T-test showed that nano emulsion significantly improved antioxidant and antibacterial activities against S. Aureus and E. Coli with p value 0.001, 0.003, and 0.000 respectively. The results prove that Nano emulsion is potential for improving lipophilic drugs

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