Pain killers are those drugs that can reduce pain. Some of these agents have been recorded to affect bone structure. The aim is to study Diclofnac, Piroxicam, and Tramadol effects on the density of bone using the DEXA scan and to compare these effects. Sixteen sheep were incorporated in our present study. The sample study was divided randomly into four groups; each group includes 4 sheep. Group1: (control group). Group 2: diclofenac sodium injection / IM at 1mg /kg/ twice daily. Group 3: received IM injection of piroxicam at 0.5 mg /kg/ day. Group 4: received IM injection of tramadol at 5mg /kg/ day. These treatments continued for 140 days. At the slaughter of the animals, 32 femur samples (8 samples/ group) be taken for DEXA analysis. The measurements were displayed as control (0.84 ± 0.02), diclofenac (0.28 ± 0.034), piroxicam (0.67 ± 0.35) and tramadol (0.89 ± 0.26) groups in unite of gm / cm2 using the DEXA technique. Were it being equal to control (162.2 ± 0.78) mg/cm2, diclofenac (157.5 ± 2.76) mg/cm2, piroxicam (161.9 ± 0.80) mg/cm2 and tramadol groups (178.8 ± 0.46) mg/cm2 by orthopantomography.