This study was conducted to analysis the effect of Time-OF-Day on physical fitness components for students of Physical Education Department-Duhok University.The aim of this study was to compare the level of some physical fitness components between students of the morning shift and evening shift studies of the first stage in the college of Physical Education, The selected variables for the study were speed by (sprint 60 M test), abdominal endurance by (Abdominal muscles test) , anaerobic power and agility by (5-m multiple shuttle run test) and explosive strength of the arms by (Medicine ball throw test) of both groups were compared by using the statistical package for Scientific Sciences SPSS. The data of this study is not normally distributed. Therefore, non-parametric methods were used to analyze this data (Chi-square and Phi methods). Statistical analysis methods were applied to compare between the students’ level in the morning shift and evening shift. When the data were collected for both shift from the physical education department in Duhok University. significant relation was found between all variables and Time-OF-Day except the year of study as there is no significant relation between Time-OF-Day and the year of study