Waste cooking oil is one of the vegetable oils produced from the use of cooking oil continuously and is very dangerous if consumed again because it has exceeded the maximum level of quality of cooking oil, one way to utilize triglycerides contained in waste cooking oil is by transesterifying the waste cooking oil with methanol into biodiesel using heterogeneous catalysts. The purpose of this study was to determine the effect of adding the concentration of CaO/ZnO catalyst, mole ratio of waste cooking oil and methanol, and reaction time to the yield, and characteristics of biodiesel. The production of biodiesel was carried out by varying the concentration of CaO/ZnO catalysts by 0.5 %, 1 %, 1.5%, 2 %, and 2.5 % w/w oil, the mole ratio of oil with methanol 1 : 3, 1 : 6, 1 : 9, 1 : 12, and 1 : 15, reaction times of 2, 3, and 4 hours respectively. In this study, the best catalyst concentration was 1% w/w oil with the mole ratio of oil with methanol 1: 12, and reaction time of 4 hours with yield of 80.90 %, The results of the characteristics analysis of biodiesel obtained have met the SNI 7182: 2015 standard